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Literature summary extracted from

  • Timar, K.K.; Junnikkala, S.; Dallos, A.; Jarva, H.; Bhuiyan, Z.A.; Meri, S.; Bos, J.D.; Asghar, S.S.
    Human keratinocytes produce the complement inhibitor factor I: Synthesis is regulated by interferon-gamma (2007), Mol. Immunol., 44, 2943-2949.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.45 medicine studies on mutations of complement factor I Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.45 expressed in Escherichia coli, mutant and unmodified proteins Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.45 G243D cofactor function of complement components C3b and Cb4 not affected Homo sapiens
3.4.21.45 M120I clinical data of aHUS-patients with complemment factor I mutation summarized, cofactor function of complement components C3b and Cb4 not affected Homo sapiens
3.4.21.45 R388H cofactor function of complement components C3b and Cb4 not affected Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.45 50000
-
 heavy chain, indicative of processing Homo sapiens
3.4.21.45 88000
-
 non-reduced form, SDS-PAGE Homo sapiens
3.4.21.45 88000
-
 reduced form, RRKR-linker peptide not cleaved, corresponds to pro-complemement factor I Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.45 complement component C3b + H2O Homo sapiens
-
 complement component C3c + ?
-
 ?
3.4.21.45 complement component C4b + H2O Homo sapiens
-
 complement component C4c + C4d
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.45 Homo sapiens P05156 encoded by CFI gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.45 recombinant and native proteins, gel filtration, SDS-PAGE Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.45 keratinocyte incubation of foreskin with thermolysin followed by trypsinization Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.45 additional information
-
 keratinocytes stimulated with IFN-gamma (10-1000 U/ml), IL-1alpha (25-200 U/ml), IL-6 (250-1000 U/ml), TNF-alpha (100-1000 U/ml) or TGF-beta1, presence of complement factor I analyzed by monitoring cleavage of complement component C3b, assay with radiolabelled purified complement component C3b Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.45 complement component C3b + H2O
-
 Homo sapiens complement component C3c + ?
-
 ?
3.4.21.45 complement component C3b + H2O complement factor I production of human keratinocytes analyzed, with and without stimulation by IFN-gamma, IL-1alpha, IL-6, TNF-alpha, TGF-beta1 Homo sapiens complement component C3c + ?
-
 ?
3.4.21.45 complement component C4b + H2O
-
 Homo sapiens complement component C4c + C4d
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.45 37
-
 C3b cleavage assay at Homo sapiens